Understanding cell behavior in cultivation processes - A metabolic approach
نویسندگان
چکیده
Background During cultivation cells undergo a tremendous change in their metabolism when shifting from one state to another or when parameters are changed. To understand the changes in intracellular metabolite concentrations and their impact on cell performance we used a systematic approach. By employing the chemostat mode at different steady state conditions we investigated the alterations of the concentrations of key metabolites during cultivations of a human production cell line.
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